| Item # | Pack Size | Storage |
|---|---|---|
| 1-DF2-001 | 96 wells/kit | Store at 2-8℃ |
ELISA kit for quantification of the group 2 allergen from house dust mites (Dermatophagoides farinae), Der f 2.
The pre-blocked and coated microplates with capture antibodies and
the use of enzyme-labeled detection antibodies enable easy and rapid measurement.
* Der f 2 concentration in samples including Der f 2 and Der p 2 cannot be precisely quantified with this kit which has cross-reactivity to Der f 2 and Der p 2 both, and a standard for Der f 2 were used in allergen quantification.
・Measurement of Der f 2 in house dust
・Measurement of Der f 2 in bedding
・Measurement of Der f 2 in foods
・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)
| Component | Pack size |
|---|---|
| Capture antibody coated microplates | 2 packs（8 wells ×6 strips/pack） |
| Der f 2 Standard (lyophilized) * The quantity varies from lot to lot. | For 4 measurements |
| Enzyme-labeled anti-Der f 2 antibody | 12mL × 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution | 12mL × 1 |
| Stop solution（0.5M sulfuric acid） | 12mL × 1 |
| Dilute solution （for sample and reagent） | 30mL × 2 |
| Wash solution (20x concentrated) | 30mL × 1 （for 600 mL） |
| Microplate seal | 3 |
Instruction |
1 |
* Der f 2 concentration in samples including Der f 2 and Der p 2 cannot be precisely quantified with this kit which has cross-reactivity to Der f 2 and Der p 2 both, and a standard for Der f 2 were used in allergen quantification.
| Measurement range | 0.08 - 10 ng/mL |
|---|---|
| Detection limit | |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜6％ |
|---|---|
| Between-day imprecision | CV ＜6％ |
| Intermediate precision | CV ＜8％ |
Der f 2 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
1) Yasueda H, et al. Species-specific measurement of the second group of Dermatophagoides mite allergens, Der p 2 and Der f 2, using a monoclonal antibody-based ELISA. Clin Exp Allergy 1996; 26(2): 171–177.[Pubmed]
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All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
