| Item # | Pack Size | Storage | Price |
|---|---|---|---|
| 10203 | 96 wells/kit | Store at 2-8℃ | 80,000 |
ELISA kit for quantification of OVA（Ovalbumin） from chickens. The pre-blocked and coated microplates with capture antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement. It is useful for experiments on OVA kinetics in vivo.
・Quick measurement: test results in 2 hours and 15 minutes
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Quantification of OVA reagents for experiment
・Evaluation of transfected OVA expression
・Determination of OVA concentrations in blood and milk
| Component | Pack size |
|---|---|
| Pre-blocked and coated microplates with capture antibodies, 6 strips of 8 wells | 2 packs |
| Standard (lyophilized) for 4 measurements | 1 |
| Enzyme-labeled anti-OVA antibody, 12 mL | 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL | 1 |
| Stop solution, 12 mL | 1 |
| Dilute solution, 30 mL | 2 |
| Washing solution (20x concentrated), 30 mL | 1 |
| Microplate seal | 3 |
| Measurement range | 50-0.78 ng/mL |
|---|---|
| Detection limit | 0.78 ng/mL |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜3％ |
|---|---|
| Between-day imprecision | CV ＜14％ |
| Intermediate precision | CV ＜14％ |
OVA sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
| Sample | Dilution ratio | Recovery rate（%） | |
|---|---|---|---|
| Normal serum | Mouse | 1:10 | 96.5 |
| Rat | 1:10 | 97.6 | |
| Human | 1:10 | 94.5 | |
| Rabbit | 1:10 | 103.5 | |
| Dog | 1:10 | 103.1 | |
| Bovine fetus | 1:10 | 105.2 | |
| cell culture medium (10% bovine fetus serum added) |
RPMI | 1:10 | 99.5 |
| DMEM | 1:10 | 103.7 | |
Each serum and cell culture mediam (RPMI and DMEM with 10% bovine fetus serum) were used as samples.
It was shown that dilution of each sample by 10 times or more was sufficient to measure OVA accurately.
Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN. Front. Immunol. 10, 1450 (2019). [Pubmed, Fulltext]
Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN. Front. Immunol. 10, 1450 (2019). [Pubmed, Fulltext]
Stable Immune Response Induced by Intradermal DNA Vaccination by a Novel Needleless Pyro-Drive Jet Injector. AAPS PharmSciTech 21, 19 (2019). [Pubmed, Fulltext]
Systemic DC activation modulates the tumor microenvironment and shapes the long-lived tumor-specific memory mediated by CD8+ T cells. Cancer Res. 76, 3756–3766 (2016).[Pubmed]
Topical skin treatment with Fab fragments of an allergen-specific IgG1 monoclonal antibody suppresses allergen-induced atopic dermatitis-like skin lesions in mice. Eur. J. Pharmacol. 779, 131–137 (2016). [Pubmed]
Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model. Sci. Rep. 5, 1–8 (2015). [Pubmed, Fulltext]
Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells. Cancer Res. 73, 62–73 (2013). [PubMed, Fulltext]
＜Literature on this kit overview＞
Development of an enzyme-linked immunosorbent assay kit for measuring ovalbumin concentration in biomaterials.
The Allergy in practice. 2012; 32: 177-180.
All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
