| Item # | Pack Size | Storage | Price |
|---|---|---|---|
| 10204 | 96 wells/kit | Store at 2-8℃ | 60000 |
ELISA kit for quantification of the major allergen from Cryptomeria japonica, Cry j 1.
The pre-blocked and coated microplates with capture antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement.
・High sensitivity: the detection limit is 0.16 ng/mL.
・Quick measurement: test results in 2 hours and 15 minutes
・High specificity: Cry j 1 specific, having no cross reactivity with Cry j 2
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Control of the pollen lots
・Measurement of Cry j 1 in house dust
・Measurement of Cry j 1 in the air
・Evaluation and control of Cry j 1 concetration in a chamber that has been scattered
・Development and evaluation of products against the pollen (filters, air purifiers, cleaners and so on)
| Component | Pack size |
|---|---|
| Pre-blocked and coated microplates with capture antibodies, 6 strips of 8 wells | 2 packs |
| Standard (lyophilized) for 2 measurements | 2 |
| Enzyme-labeled anti-Cry j 1 antibody, 12 mL | 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL | 1 |
| Stop solution, 12 mL | 1 |
| Dilute solution, 30 mL | 2 |
| Washing solution (20x concentrated), 30 mL | 1 |
| Microplate seal | 3 |
| Measurement range | 10-0.16 ng/mL |
|---|---|
| Detection limit | 0.16 ng/mL |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜3％ |
|---|---|
| Between-day imprecision | CV ＜7％ |
| Intermediate precision | CV ＜8％ |
Cry j 1 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
Cry j 1 was specifically measured and Cry j 2 (0.1、1、10、100 ng/mL) was no cross with in this assay.
Terumi Midoro-Horiuti et al. “Structural basis for epitope sharing between group 1 allergens of cedar pollen.” Mol Immunol 43 (2006): 509-518.[PubMed]Sakaguchi, Masahiro et al. “Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.” Immunology 91.2 (1997): 161-6. [PubMed]
高橋裕一、宮澤博、阪口雅弘、井上栄、片桐進、名古屋隆生、渡辺雅尚、谷口美文、栗本雅司、安枝浩, 室内塵中のCryj1量と空中スギ花粉数との関係、アレルギー 1994; 43（2）:97-100.
渡辺雅尚、田村正広、名古屋隆生、高橋裕一、片桐進、岡鉄雄, スギ花粉抗原（CryjI）に対するモノクローナル抗体を用いたEnzyme-LinkedImmunosorbentAssay（ELISA）によるCryjIの測定、アレルギー 1992; 41（11）: 1535-1539.
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All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
